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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Estrogen-ERα signaling and DNA hypomethylation co-regulate expression of stem cell protein PIWIL1 in ERα-positive endometrial cancer cells

Fig. 6

Cancer-linked hypomethylation of the PIWIL1 promoter. a Results of bisulfite DNA sequencing of the PIWIL1 upstream regulatory region in Ishikawa, RL95–2 and HEC-1B cells. Black dots symbolize methylated CpGs and white dots symbolize unmethylated CpGs. b The percentage of methylated CpG dinucleotides in Ishikawa, RL95–2 and HEC-1B cells. ***p < 0.001 and ns, not significant. c RT-qPCR and western blot showed changes in PIWIL1 mRNA and protein expression in HEC-1B cells after treatment with 5-aza-dC. Data were represented as means ± SD for three independent experiments (RT-qPCR). ****p < 0.0001 versus control group. GAPDH was used as an internal control (western blot)

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Cell Communication and Signaling

ISSN: 1478-811X