Fig. 2
Involvement of the ERα in E2-induced PIWIL1 expression. a and b The ERα antagonist ICI 182,780 (10− 7 mol/L) was used to examine ERα in E2-induced PIWIL1 induction in Ishikawa and RL95–2 cells. c Ishikawa and RL95–2 cells were transfected with ERα shRNA (shERα) and HEC-1B was transfected with ERα expressing vector (exERα). The mRNA and protein levels of PIWIL1 and ERα were then assayed using RT-qPCR and western blot. Data were represented as means ± SD for three independent experiments (RT-qPCR). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 and ns, not significant versus control group. GAPDH was used as an internal control (western blot)