Fig. 4

Lin28a is directly up-regulated by Wnt/β-catenin and participates in implantation. a Lin28a expression in pre-and post-implantation Day 5 to Day 7 mouse embryo. Lin28a is in green, nuclei is in blue, pictures were captured under 40X objective in confocal 700, scale bar was shown as 20 μm. TE: trophectoderm, EXE: extra-embryonic ectoderm, EPI: epiblast. IHC scale bar 30 μm at 20X, 150 μm at 100 X. b Lin28a mRNA level in Wnt activation and inhibition embryo. c, d The PCR result of CHIP binding site. 5 sites have been newly detected, 2 sites were published sites (Pub 1 and 4). e The relative expression of lin28a mRNA in lin28a-TG mice embryos after doxycycline induction for 4 days. f, g In vitro attachment/outgrowth rate of lin28a-TG mouse embryos on ME cells at 24 h. h, i The outgrowth status and area of lin28a-TG embryos. Data are means ± s.d. (N = 3–5), bar = 100 μm in h, p < 0.05 “*”, p < 0.01 “**”