Fig. 2

Repression of let-7a/g by Wnt/β-catenin pathway in mouse embryo. a Expression of mature let-7a/g in pre-implantation embryo from 2 cell stage to blastocyst, mir-16 was used as internal control. b The location of active-β-catenin in embryo upon Wnt activation or inhibition. CHIR99021(10 μM) and DKK (1 μg/ml) treated for 24 h. (Arrow: nuclear active-β-catenin) (c) qPCR analysis of the mature let-7a/g in blastocysts with Wnt activation by CHIR99021 or Wnt inhibition by DKK for 24 h. Data are means ± s.d. (N = 5), bar = 20 μm, p < 0.05 “*”, p < 0.01 “**”