Fig. 8
From: TRPP2 and STIM1 form a microdomain to regulate store-operated Ca2+ entry and blood vessel tone
Generation and verification of the TRPP2 conditional knockout (CKO) mice. a Schematic description of gene-targeting map of PKD2 gene. Targeting construct is referring to the floxed allele. Flp-recombined is obtained from deletion of the Neomycin cassette. Cre-recombined is the mutant allele yielded from the deletion with Cre-recombinase. b Genomic PCR analysis data from mice tail DNA samples showing the presence of LoxP+/+ sites and Cre-transgens. c Immunoblots data showing TRPP2 expressing in aortic smooth muscle cells from TRPP2 CKO or Cre control mice. d, e Agonist-induced mouse aorta contraction. Phenylephrine (10 μmol/L, d) and endothelin 1 (100 nmol/L, e) concentration-dependently induced the contraction of TRPP2 CKO or Cre control mice aortae. Values are shown as mean ± SEM (n = 4–8 mice). *P < 0.05 for Cre control vs. TRPP2 CKO mouse