Fig. 6

Role of TRPP2 and STIM1 in Ca²⁺ release and store-operated Ca²⁺ entry (SOCE)-induced mouse aorta contraction. a Representative traces showing phenylephrine (Phe, 10 μmol/L)-induced contraction in Ca²⁺-free solution and extracellular Ca²⁺ (2.5 mmol/L) re-addition-induced contraction in mice aortae. b-e Summarized data showing Phe-induced contraction in Ca²⁺-free solution (b, d) and extracellular Ca²⁺ re-addition (c, e)-induced contractions in mice aortae, which were transfected with TRPP2 siRNA (b, c), SITM1 siRNA (d, e) or scrambled siRNA. f-i Summarized data showing endothelin 1 (ET-1, 100 nmom/L)-induced contraction in Ca²⁺-free solution (f, h) and extracellular Ca²⁺ re-addition (g, i)-induced contractions in mice aortae transfected with TRPP2 siRNA (f, g), SITM1 siRNA (h, i) or scrambled siRNA. Values are shown as mean ± SEM (n = 3–4 mice). ^*P < 0.05 for scrambled siRNA vs. TRPP2 siRNA or STIM1 siRNA transfection. j Representative traces showing thapsigargin (TG, 2.5 μmol/L)-induced contraction in Ca²⁺-free solution and extracellular Ca²⁺ (2.5 mmol/L) re-addition-induced contraction in mice aortae. k-n Summarized data showing TG-induced contraction in Ca²⁺-free solution (k, m) and extracellular Ca²⁺ re-addition (l, n)-induced contraction in mice aortae, which were transfected with TRPP2 siRNA (j-l), SITM1 siRNA (m-n) or scrambled siRNAs. Values are shown as the mean ± SEM (n = 3–4 mice). ^*P < 0.05 for TRPP2 siRNA or STIM1 siRNA vs. scrambled siRNA