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Table 1 Primers

From: Protease associated domain of RNF43 is not necessary for the suppression of Wnt/β-catenin signaling in human cells

Cloning and mutagenesis primers

Primer

Sequence

Purpose

RNF43 InFusion F

GTTTAAACTTAAGCTTATGAGTGGTGGCCACCAG

RNF43-BirA*-HA into pcDNA4

RNF43 InFusion R

AAACGGGCCCTCTAGACTATGCGTAATCCGGTACA

del87–186

ATTAATGCAGTCCCACGAGCTGAAGGAGCCCC

Site-directed mutagenesis, deletion of the PA domain

del87–186-antisense

GGGGCTCCTTCAGCTCGTGGGACTGCATTAAT

Quantitative Polymerase Chain Reaction

GENE

Forward primer seq.

Reverse primer seq.

Product size

B2M

CACCCCCACTGAAAAAGATG

ATATTAAAAAGCAAGCAAGCAGAA

167

GAPDH

GACAGTCAGCCGCATCTTCT

TTAAAAGCAGCCCTGGTGAC

127

RNF43

TTTCCTGCCTCCATGAGTTC

CAGGGACTGGGAAAATGAATC

116

ZNRF3

GCTTTCTTCGTCGTGGTCTC

GCCTGTTCATGGAATTCTGAC

91

CTNNB1

AAAGCGGCTGTTAGTCACTGG

CGAGTCATTGCATACTGTCCAT

215

AXIN2

TACACTCCTTATTGGGCGATCA

TTGGCTACTCGTAAAGTTTTGGT

151

CRISPR/Cas9 Genotyping

Primer

Sequence

Purpose

RNF43 F

AGGTCTTTTACAAGTAGATAATAGCAAGG

CRISRP/Cas9 RNF43 RFLP analysis

RNF43 R

GCTGCAACCCTTTCCCA

ZNRF3 F

TTCTCAGCCTTGCTCTTCTG

CRISRP/Cas9 ZNRF3 RFLP analysis

ZNRF3 R

TCACCCTGGCCTTGAGTC