Skip to main content
Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Blocking Connexin-43 mediated hemichannel activity protects against early tubular injury in experimental chronic kidney disease

Fig. 2

ATPγS regulates the expression of major adherens and tight junction proteins in cultured human renal tubule cells. Cells were cultured in low glucose control (0) ± ATPγS (1-100 μM) for 48 h. Whole-cell expression of E-cadherin (a), N-cadherin (b), β-catenin (c), Claudin-2 (d) and ZO-1 (e) were assessed through immunoblotting. Representative blots for each protein are shown, with expression normalized by re-probing for ɑ-tubulin as a loading control. Bars correspond to their associated lanes in the respective blot. Results were from three or more separate experiments; with key significance shown: *P < 0.05; **P < 0.01; ***P < 0.001

Back to article page