Fig. 2From: Blocking Connexin-43 mediated hemichannel activity protects against early tubular injury in experimental chronic kidney diseaseATPγS regulates the expression of major adherens and tight junction proteins in cultured human renal tubule cells. Cells were cultured in low glucose control (0) ± ATPγS (1-100 μM) for 48 h. Whole-cell expression of E-cadherin (a), N-cadherin (b), β-catenin (c), Claudin-2 (d) and ZO-1 (e) were assessed through immunoblotting. Representative blots for each protein are shown, with expression normalized by re-probing for ɑ-tubulin as a loading control. Bars correspond to their associated lanes in the respective blot. Results were from three or more separate experiments; with key significance shown: *P < 0.05; **P < 0.01; ***P < 0.001Back to article page