Fig. 6

Wnt5a is crucial for the pro-tumor functions of TAMs. a Western blot analysis of TAMs with or without Box 5. b Western blot analysis of TAMs with or without U0126 or S3I-201. c Western blot analysis of TAMs with or without CK59. d ELISA detected the secretion of IL-10 in TAMs treated with inhibitors. Error bars, SEM. e Flow cytometry analysis of TAMs with or without inhibitors. Error bars, SEM. f, g Representative photographs and quantification of clone formation assay in HCT116 and DLD-1 cells co-cultured with M0 macrophages and TAMs transfected with sh-Wnt5a or sh-NC. Bar = 0.5 cm. Error bars, SEM. h, i Transwell migration assay of HCT116 and DLD-1 cells co-cultured with conditioned macrophages. Error bars, SEM. Bar = 200 μm. j, k Transwell invasion assay of HCT116 and DLD-1 cells co-cultured with conditioned macrophages. Error bars, SEM. Bar = 200 μm. All experiments were performed in triplicate. Statistical analysis was conducted using one-way ANOVA. *P < 0.05. **P < 0.01. ***P < 0.001