Fig. 5From: Wnt5a-induced M2 polarization of tumor-associated macrophages via IL-10 promotes colorectal cancer progressionWnt5a indirectly promotes tumor proliferation, migration and invasion through M2 macrophages. a Flow chart of CRC cells co-cultured with M0 macrophages treated with Wnt5a or Wnt5a + IL-10 antibody or Wnt5a + IgG. b, c Representative photographs and quantifications of clone formation assay in HCT116 and DLD-1 cells co-cultured with conditioned M0 macrophages. Bar = 0.5 cm. d, e Transwell migration assay of HCT116 and DLD-1 cells co-cultured with conditioned M0 macrophages. Bar = 200 μm. f, g Transwell invasion assay of HCT116 and DLD-1 cells co-cultured with conditioned M0 macrophages. Bar = 200 μm. All experiments were performed in triplicate. Statistical analysis was conducted using one-way ANOVA. Error bars, SEM. ns, not significant. ***P < 0.001Back to article page