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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Wnt5a-induced M2 polarization of tumor-associated macrophages via IL-10 promotes colorectal cancer progression

Fig. 4

Wnt5a up-regulates IL-10 expression by activating CaKMII-ERK1/2-STAT3 pathway. a Western blot analysis of p-ERK1/2, p-STAT3 and p-NF-κB p65 expression in M0 macrophages with or without Wnt5a. b Immunofluorescence staining for ERK1/2 and STAT3 in M0 macrophages with or without Wnt5a. Bar = 25 μm. c Western blot analysis of IL-10, p-ERK1/2 and p-STAT3 expression in Wnt5a-treated M0 macrophages with or without U0126 or S3I-201. d Flow cytometry analysis of Wnt5a-treated M0 macrophages with or without U0126 or S3I-201. Error bars, SEM. e ELISA analysis of IL-10 secretion level in Wnt5a-treated M0 macrophages with or without U0126 or S3I-201. Error bars, SEM. f Western blot analysis of Wnt5a-treated M0 macrophages with or without CK59 or U0126 or S3I-201. g ELISA analysis of IL-10 secretion level in Wnt5a-treated M0 macrophages with or without CK59. Error bars, SEM. h Flow cytometry analysis of Wnt5a-treated M0 macrophages with or without CK59. Error bars, SEM. All experiments were performed independently at least three times. Statistical analysis was conducted using one-way ANOVA. ***P < 0.001

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Cell Communication and Signaling

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