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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Wnt5a-induced M2 polarization of tumor-associated macrophages via IL-10 promotes colorectal cancer progression

Fig. 2

Wnt5a is mainly expressed in M2-like TAMs. a Flow chart of generating TAMs. b Relative expression of M1 markers (HLA-DR, IL-12), M2 markers (Arg-1, CD163, CD206, IL-10, TGFβ, CCL17, CCL18, CCL22) in M0 macrophages, M2 macrophages and TAMs co-cultured with HCT116 or DLD-1 for 48 h. Error bars, SEM. c Flow cytometry analysis of the proportion of M2 cells in different groups of macrophages. Error bars, SEM. d The expression level of Wnt5a in M0, M1, M2 macrophages and TAMs co-cultured with HCT116 or DLD-1 for 48 h. e ELISA analysis of Wnt5a secretion level in macrophages, CRC cell lines and CRC cell lines co-cultured with macrophages. Error bars, SEM. f Representative immunofluorescence photographs for Wnt5a, CD163 and DAPI in different groups of macrophages. Bar = 50 μm. All experiments were performed independently at least three times. Statistical analysis was conducted using one-way ANOVA. *P < 0.05. **P < 0.01. ***P < 0.001

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