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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Low DLG2 gene expression, a link between 11q-deleted and MYCN-amplified neuroblastoma, causes forced cell cycle progression, and predicts poor patient survival

Fig. 5

Cell responses after DLG2 over expression or silencing. Cell responses 48 h post DLG2 over expression (DLG2-GFP) or silencing (si-DLG2) in SKNAS cells (11q-deleted): a proliferation; b viable and non-viable cell fraction; c cell cycle analysis. Cell responses 48 h post DLG2 over expression (DLG2-GFP) or silencing (si-DLG2) in NB69 cells (11q-normal): d proliferation; e viable and non-viable cell fraction; f cell cycle analysis. DLG2 gene expression analysis 48 h post DLG2 over expression (DLG2-GFP) or silencing (si-DLG2) in g SKNAS cells; h NB69 cells. idmDLG gene expression in wild type and dmDLG silenced (RNAi-dmDLG) Drosophila larvae. j immunoblot of wild type and dmDLG silenced (RNAi-dmDLG) Drosophila larvae targeting dmDLG, Cyclin A, Cyclin B, normalized to α-tubulin. The data in a-f shows the mean and SD. a and d are normalized as a percentage of the mock transfection, n = 9. The data shown is the pooled average of 3 experiments. g and h are expressed as median centered log2 fold change and plotted as Tukeys box and whisker plots showing IQR, line at the median, + at the mean with whiskers ±1.5-fold of interquartile range. *p < 0.05, ***p < 0.001, n = not significant

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Cell Communication and Signaling

ISSN: 1478-811X