Fig. 3

DLG2 expression in MYCN amplified NB. DLG2 expression level and MYCN status in primary NB a NB primary dataset 1 (GSE49710) and b NB primary dataset 2 (GSE16476); and in NB cell line datasets c NB cell dataset 1 (GSE89413) and d NB cell dataset 2 (GSE28019), bone marrow derived. e The effect of MYCN induction by doxycycline on DLG2 expression in SKNAS cells over time from NB cell dataset 3 (GSE16478). The data (a-d) are presented as median centered log2 fold change and plotted as Tukeys box and whisker plots showing IQR, line at the median, + at the mean with whiskers ±1.5-fold of interquartile range. Data outside the whiskers are shown as outliers. fdmDLG gene expression in wild type and dMYC over expressing (UAS-dMyc) Drosophila larvae. g immunoblot of wild type and dMYC over expressing (UAS-dMyc) Drosophila larvae targeting dmDLG, Cyclin A, Cyclin B normalized to α-tubulin. e and f are expressed as the mean ± SEM. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001