Table 1. Primer sets
Gene | Forward primer | Reverse primer | Annealing temperature |
|---|---|---|---|
ATF3 | 5’-CTG CAG AAA GAG TCG GAG-3’ | 5’-TGA GCC CGG ACA ATA CAC-3’ | 53 °C |
COX2 | 5’-GAA TGG GGT GAT GAG CAG TT-3’ | 5’-CAC AAG GGC AGG ATA CAG C-3’ | 52 °C |
BTF3 | 5’-AGC TTG GTG CGG ATA TGA-3’ | 5’-GTG CTT TTC CAT CCA CAG ATT G-3’ | 55 °C |
SNAIL1 | 5’-GAA AGG CCT TCA ACT GCA AA-3’ | 5’-TGA CAT CTG AGT GGG TCT GG-3’ | 56 °C |
ZFP91 | 5’-AGC TAC CAT TTG CCT ACA A-3’ | 5’-GGG AAA CGG CTG AGA TAG TTT-3’ | 43 °C |
LRH1 | 5’-GCA TCT TGG GCT GCC TGC AG-3’ | 5’-CCT TGC CGT GCT GGA CCT GG-3’ | 62 °C |
Sp1 | 5’-GCC TCC AGA CCA TTA ACC TCA GT-3’ | 5’-GCT CCA TGA TCA CCT GGG GCA T-3’ | 61 °C |
p21 | 5’-GTC CGT CAG AAC CCA TGC-3’ | 5’-GTC GAA GTT CCA TCG CTC A-3’ | 56 °C |
GAPDH | 5’-TGG GCT ACA CTG AGC ACC AG-3’ | 5’-GGG TGT CGC TGT TGA AGT CA-3’ | 55 °C |