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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Regulators of G-protein signaling, RGS2 and RGS4, inhibit protease-activated receptor 4-mediated signaling by forming a complex with the receptor and Gα in live cells

Fig. 1

Interactions between PAR4 and either RGS2 or RGS4 in live cells. (Insets of a, c and e) Schematic depiction of fusion and untagged proteins used in the BRET experiment. a, c BRET analysis of 293 T cells co-transfected with PAR4-Venus (0, 0.1, 0.25, 0.5, 0.75, 1.0 μg), either RGS2-Luc (0.1 μg) (a) or RGS4-Luc (0.1 μg) (c), and 0.5 μg of the indicated untagged GαEE. b, d BRET analysis of 293 T cells transfected with PAR4-Venus (1.0 μg), either RGS2-Luc (0.1 μg) (b) or RGS4-Luc (0.1 μg) (d), and 0.5 μg of the indicated untagged GαEE. e BRET analysis of cells were transfected with 1 μg PAR4-Venus, 0.1 μg RGS-Luc, and 0.5 μg indicated untagged GαEE in the presence and absence of AYPGKF, a PAR4 agonist. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, compared to controls. All BRET results are representative of at least three independent experiments

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Cell Communication and Signaling

ISSN: 1478-811X