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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: MicroRNA-153 impairs presynaptic plasticity by blocking vesicle release following chronic brain hypoperfusion

Fig. 4

MiR-153 represses synaptic vesicle exocytosis by targeting vesicle release-related proteins. a FM1–43 fluorescent dye images showing presynaptic boutons in neurons before stimulation (t = 0 s) and following PBS or 70 mM KCl stimulation at 10, 20, 30, and 40 s. The insert shows enlarged synaptic boutons from the framed area in the main panels. b FM1–43 fluorescent dye imaging analysis (χ2Mauchly = 3068.620, P < 0.0001; Ftotal(39, 2146) = 69.973, P < 0.0001). c Bar graph of FM1–43 staining normalized fluorescence density at 10 s (PLevene = 0.330, one-way ANOVA: F = 46.738, P < 0.0001) and 40 s (PLevene = 0.105, one-way ANOVA: F = 90.788, P < 0.0001) following 70 mM KCl stimulation. n = 17 synaptic boutons from 3 batches of NRNs in each group. d-h FM1–43 fluorescence intensity curve showing the action of Snap25-ODN (d, Ftotal (39, 1132) = 27.987, P < 0.0001), Vamp2-ODN (e, Ftotal (39, 1132) = 32.628, P < 0.0001), Stx1a-ODN (f, Ftotal (39, 1132) = 40.414, P < 0.0001), Syt1-ODN (g, Ftotal (39, 1132) = 32.183, P < 0.0001) or the four ODNs combined (h, Ftotal (39, 2029) = 25.076, P < 0.0001) on FM1–43 fluorescent dye exocytosis. i-k Statistical analysis of the effects of the SNAP-25, VAMP-2, syntaxin-1A and Syt1 proteins on vesicle release. Bar graph showing the percentage of released vesicles after 70 mM KCl stimulation at 5 s (i: PLevene = 0.357, one-way ANOVA: F = 15.657, P < 0.0001), 10 s (j: PLevene = 0.027, one-way ANOVA: F = 24.181, P < 0.0001) and 40 s (k: PLevene = 0.142, one-way ANOVA: F = 33.132, P < 0.0001). n = 12 synaptic boutons from 3 batches of NRNs in each group. Fisher’s PLSD test was used for the post hoc analyses of the two-group comparisons. *P < 0.05 vs NC; #P < 0.05 vs miR-153

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