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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: A cancer stem cell-like phenotype is associated with miR-10b expression in aggressive squamous cell carcinomas

Fig. 4

Gap closure is affected by miR-10b expression. a Stable overexpression of miR-10b in an E6/E7 immortalized RDEB keratinocyte line (RDEB-KCmiR-10b) prolonged the time to gap closure compared to parental cells (RDEB-KCE6/E7; n ≥ 8 ind. experiments). b Knock-out of MIR10B in RDEB-SCC1 (RDEB-SCC1MIR10B−/−) enhanced gap closure compared to parental cells (RDEB-SCC1), whereas a mixed culture of miR-10b expressing cells with RDEB-SCC1MIR10B−/− resembled parental results (n ≥ 3 ind. experiments). c Transient transfection of a miR-10b mimic into RDEB-SCC1MIR10B−/− decelerated gap closure in knock-out cells compared to scrambled control (two ind. experiments with n = 4 technical replicates each, one representative run shown). d Gap closure of three HC-cSCC and (e) three RDEB-SCC cell lines compared to primary HC-KC (1090KC; n = 3 independent experiments). Data represented by mean ± SEM, *p < 0.05, n.s. non significant, unpaired t-test. Representative images are shown for either time points until gap closure. Red line indicates six hour time point in each experiment

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