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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: AKT2 deficiency impairs formation of the BCR signalosome

Fig. 3

BCR signaling is decreased in AKT2 KO mice. The BCR signal molecular is detected by CFm (a, b) and flow cytometry (d). Spleen cells from WT and AKT2 KO mice (n = 3) were loaded on lysine-coated slides and incubated with AF546-mB-F (ab)2′-anti-Ig without (−) or with streptavidin, and then stimulated in 37 °C for indicated times. After fixed and permeabilized, cells were stained with anti-pY antibody and analyzed with CFm. The images of CFm are shown (a, b), and the Pearson’s correlation coefficients between BCR and pY in stimulated cells were determined by NIS-Elements AR 3.2 software (c). Data were generated from more than 50 cells from 3 independent experiments. Scale bars, 2.5 μm. Flow cytometry analysis of the MFI of pY from stimulated cells at different time points (d), and the average value (± SEM) was presented from 3 individual experiments, *p < 0.05

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