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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: TRAF1 suppresses antifungal immunity through CXCL1-mediated neutrophil recruitment during Candida albicans intradermal infection

Fig. 8

TRAF1-deficient macrophages play a critical role in C. albicans skin infection in vivo. a. WT mice (n = 8, 6 weeks old) and Traf1−/− (n = 4, 6 weeks old) recipient mice were fed with antibiotics in the drinking water for 2 weeks and were lethally irradiated. In 4 h, bone marrow cells (BM) from WT or Traf1−/− donors were injected intravenously (i.v.) (1 × 106 cells /mouse) into the recipient mice. Antibiotics in drinking water were fed for another 2 weeks and then regular water was restored for another 2 weeks before challenged with C. albicans for 3 days. b. The area of the skin ulcer of reconstituted mice 3 days post-infection was measured. c. OCT-embedded skin sections from reconstituted mice 3 days post-infection were stained by PAS. Representative micrographs were captured at 50× magnification. d. WT and Traf1−/− macrophages (MФ) were injected intravenously (1 × 106 cells /mouse) into WT mice. In 4 h, recipient mice were challenged with C. albicans for 3 days. e. The area of the skin ulcer of recipient mice 3 days post-infection was measured. f. OCT-embedded skin sections from recipient mice 3 days post-infection were stained by PAS. Representative micrographs were captured at 50× magnification. Data are shown as mean ± SEM, and were analyzed using the unpaired, two-tailed, Student’s t-test. Values of p below 0.05 represented a statistically significant difference

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