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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: TRAF1 suppresses antifungal immunity through CXCL1-mediated neutrophil recruitment during Candida albicans intradermal infection

Fig. 4

TRAF1-deficiency promotes the recruitment of neutrophils but not granulopoiesis. Traf1−/− mice (n = 4) and WT controls (n = 4) were infected with C. albicans (1 × 107 CFU) or PBS for 3 days. a. Skin tissue single-cell suspensions were analyzed by flow cytometry. Representative plots were gated on neutrophils (top: CD45+CD11b+Ly6G+), monocytes (middle: CD45+Ly6G−CD11b+Ly6C+) and macrophages (bottom: CD45+CD11b+F4/80+). Cell number were calculated: the percentage of skin single cell suspensions × the number of skin single cell suspensions/the skin volume (mm3). b. Bone marrow cell suspensions were analyzed by flow cytometry. Bone marrow single live cell suspensions were Fixable Viability Dye negative. Representative plots were gated on neutrophils (CD45+CD3−B220−CD11b+Ly6G+). The percentages were calculated as percentages of total bone marrow cells. PBS group, the control group; CA group, the c. albicans infection group. Data are shown as mean ± SEM and were analyzed using the unpaired, two-tailed, Student’s t-test. Values of p below 0.05 represented a statistically significant difference. Values of p below 0.05 represented a statistically significant difference

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