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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Induction of immunogenic cell death in radiation-resistant breast cancer stem cells by repurposing anti-alcoholism drug disulfiram

Fig. 3

DSF/Cu induced a similar extent of IDC in both BCSCs and non-BCSCs. Sorted cells were treated with (0.15/1 μM) DSF/Cu for 24 h and then cells and supernatants were analyzed for ICD. (a) Apoptotic cells were quantitated (left panel). Percentage of apoptotic cells are indicated (right panel). (b) Percentages of cells expressing CRT (left panel) and their mean MFI values are indicated (right panel). (c) Percentages of cells expressing HSP90 (left panel) and their MFI values (right panel). (d) Levels of intracellular ATP were measured (left panel) and their MFI values are shown (right panel). (e) Concentrations of HMGB1 release were determined in supernatants. *indicates p < 0.05, **indicates p < 0.01 (DSF/Cu treated vs DMSO treated cells)

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Cell Communication and Signaling

ISSN: 1478-811X