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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Induction of immunogenic cell death in radiation-resistant breast cancer stem cells by repurposing anti-alcoholism drug disulfiram

Fig. 2

Radiation induced a low level of ICD in BCSCs than in non-BCSCs. Sorted cells were treated with IR at 8 or 12 Gy and cultured. Then cells and/or culture supernatants were collected at indicated times post IR and used for analyses of ICD by flow cytometry and ELISA. (a) Apoptotic cells were quantitated 24 h post-IR using AnnexinV/7-AAD staining (left panel). Percentages of apoptotic cells are indicated (right panel). (b) Percentages of cells expressing CRT were detected 8 h post IR (left panel) and their mean fluorescence intensity (MFI) values are shown (right panel). (c) Percentages of cells expressing HSP90 were detected 8 h post IR (left panel) and their MFI values are shown (right panel). (d) Levels of intracellular ATP were measured 1 h post-IR (left panel) and their MFI values are shown (right panel). (e) Concentrations of HMGB1 release were determined in supernatants collected at 24 h post-IR. *indicates p < 0.05, **indicates p < 0.01

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Cell Communication and Signaling

ISSN: 1478-811X