Fig. 5

Activation of Nrf2 induces PDGF-A expression and impairs adipogenesis in BM-MSCs. a Experimental design. Relative Nqo1 and Hmox1 (b) and Pdgfa (c) mRNA levels in t-BHQ (10 μM, 24 h) or DMSO treated growing Nck1^+/+ BM-MSCs derived from week 5 post-weaning mice (n = 4–5/group). d At day 7 of differentiation, representative images (DIC, 10X), Oil red O staining quantification (n = 3–5/group) and relative Pparg and Fabp4 mRNA levels (n = 4/group) in t-BHQ (10 μM) or DMSO treated Nck1^+/+ BM-MSCs isolated from week 5 post-weaning mice. At day 7 of differentiation, relative Nqo1 (e) and Pdgfa (f) mRNA levels in t-BHQ (10 μM) or DMSO treated Nck1^+/+ BM-MSCs isolated from week 5 post-weaning mice (n = 4/group). Data are mean ± SEM. Statistical significance evaluated by unpaired Student’s t-test and is reported as *p ≤ 0.05, and **p ≤ 0.01