Fig. 4

Activation of Nrf2 is dependent on PDGFRα. a Relative Nqo1 and Hmox1 mRNA levels in Imatinib (10 μM, 24 h) or PBS treated growing Nck1^+/+ and Nck1^−/− BM-MSCs isolated from week 5 post-weaning mice (n = 3–5/group). b Relative Nqo1 and Hmox1 mRNA levels in PDGF-AA (25 ng/mL)-stimulated (15 min, 4 and 8 h) over-night starved Nck1^+/+ BM-MSCs isolated from week 5 post-weaning mice (n = 3–4/group). Relative Nrf2 (c) and Pdgfa (d) mRNA levels in PDGF-AA (25 ng/mL)-stimulated (15 min and 4 h) over-night starved Nck1^+/+ BM-MSCs isolated from week 5 post-weaning mice (n = 4/group). Data are mean ± SEM. Statistical significance evaluated by unpaired Student’s t-test and is reported as *p ≤ 0.05, and **p ≤ 0.01