Fig. 1

Nck1 is required for differentiation of BM-MSCs into adipocytes. a Adipogenesis markers and Nck1 relative mRNA levels before (Day 0, black bars) and upon induction of adipogenesis (Day 7, white bars) in Nck1^+/+ BM-MSCs isolated from week 5 post-weaning mice (n = 4/group). b Representative images (DIC, 10X) and Oil red O staining pictures and quantification in Day 7 differentiated Nck1^+/+ and Nck1^−/− BM-MSCs isolated from week 5 post-weaning mice (n = 4/group). c Relative Pparg, Fabp4, and Adipoq mRNA levels in day 7 differentiated Nck1^+/+ (black bars) and Nck1^−/− (white bars) BM-MSCs isolated from week 5 post-weaning (W5) and week 16 post-weaning (W16) mice (n = 3–4/group). d Expression of adipogenesis markers at 7 days of differentiation as determined by western blot and densitometry relative to Hsp90 in Nck1^+/+ (black bars) and Nck1^−/− (white bars) BM-MSCs isolated from week 5 post-weaning mice (n = 3/group). Arrow represents PPARγ2. Data are mean ± SEM. Statistical significance evaluated by unpaired Student’s t-test and is reported as *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001