Fig. 3From: Cancer-associated fibroblasts promote prostate tumor growth and progression through upregulation of cholesterol and steroid biosynthesisRepresentative Western blots of HMGCS2 and AKR1C3 in various PCa cell lines after conventional 2D culture. Enzalutamide resistant sublines (EnzaR) were established through long-term culture of parental cells in the presence of 8 μM enzalutamide. Quantification of bands was performed with Image Studio (Li-Cor) and the ratio between the protein of interest and GAPDH was blotted in a graph. Data represent the mean plus SEM from at least three independent experiments. (* P < 0.05, ** P < 0.01,*** P < 0.001)Back to article page