Skip to main content
Fig. 5 | Cell Communication and Signaling

Fig. 5

From: miR-544 promotes maturity and antioxidation of stem cell-derived endothelial like cells by regulating the YY1/TET2 signalling axis

Fig. 5

Overexpression of miR-544 promotes TET2 transcription. a qPCR analysis of mRNA levels of TET genes before and after the induced differentiation of iEClCs. *p < 0.05 vs. HuAECs; t test; n = 3; b qPCR analysis of the expression level of endogenous TET2 mRNA after overexpression of miR-544. **p < 0.01 vs. miR-mut;t test;n = 3. c Western blot analysis of the expression level of TET2 in each group of cells. d The structure of PRC2. e Co-IP western blot analysis of the expression level of PRC2. *p < 0.05 vs. miR-mut; t test; n = 3. (F) The bioinformatics prediction of DNA motif sequence recognizable by YY1. g Thestructure of luciferase reporter plasmid and the results of the luciferase reporter gene assay. *p < 0.05 vs. miR-mut; t test; n = 3. (H) ChIP-PCR assay of YY1 binding to the promoter of TET2 gene. **p < 0.01 vs. miR-mut;t test;n = 3

Back to article page

Cell Communication and Signaling

ISSN: 1478-811X