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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Chitinase 3 like 1 suppresses the stability and activity of p53 to promote lung tumorigenesis

Fig. 6

Human lung cancer patients in reverse expression, intracellular colocalization, and physical interaction between Chi3L1 and p53 in (a) Normal and lung tumor extracts which isolated from lung cancer patients were analyzed by Western blotting and detected using antibodies against Chi3L1 and β-actin. b The Chi3L1 level were detected by ELISA assay in lung cancer patients and normal person serum. c Human normal lung or tumor sections (Stage I–III) were processed and stained with Hematoxylin or analyzed by immunohistochemistry for detection of positive cells for Chi3L1, p53, p21, and p-STAT3. d After human patient tissues and normal lung tissues were permeabilized, the tissues were analyzed by confocal microscopy for detection of intracellular co-localization for Chi3L1 (green) and p53 (red). And then sections were stained with DAPI (blue). Merged I shows the merged images of Chi3L1 and p53, and Merged II shows the merged images of Chi3L1, p53, and DAPI. Negative control experiments (CON) were processed with only Alex568- and Alex488-conjugated IgG antibodies. e Human patient tissues and normal lung tissues were lysed and immunoprecipitated with anti-control IgG or anti-Chi3L1 antibodies. The immunocomplexes (IP) and extracts (Input) were then analyzed by Western blotting with anti-Chi3L1 and anti-p53 antibodies

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