Fig. 4

Intracellular co-localization and physical interaction between Chi3L1 and p53 (a) After lung cancer cells were transfected with Myc-Chi3L1 for 24 h, the cells were fixed and permeabilized. Myc-Chi3L1 (green) was immunostained with mouse anti-Myc antibody, followed by Alex488-conjugated secondary antibodies. And then sections were stained with DAPI (blue). b The lung cancer cells were lysed and immunoprecipitated with anti-Myc antibody. Cell lysates (Input) and immunoprecipitants (IP) were then analyzed by Western blotting with anti-Myc and anti-p53 antibodies. c Molecular surface representation in the docking model of p53 with Chi3L1. d After the lung cancer cells were permeabilized, p53 (red) was immunostained with rabbit anti-p53 followed by Alex568-conjugated secondary antibodies and myc-Chi3L1 (green) was immunostained with mouse anti-Myc antibody, followed by Alex488-conjugated secondary antibodies. And then sections were stained with DAPI (blue). Merged I shows the merged images of Myc-Chi3L1 and p53, and Merged II shows the merged images of myc-Chi3L1, p53, and DAPI. Negative control experiments (CON) were processed with only Alex568- and Alex488-conjugated IgG antibodies. Arrow: Myc-Chi3L1-expressing cell, Arrow head: Myc-Chi3L1-non-expressing cell