Fig. 3

Regulation of Chi3L1 on lung cancer cell growth and tumor suppressor proteins, and diseases and signaling network associated with Chi3L1 and p53. a, b After A549 lung cancer cells were transfected with Chi3L1 siRNA for 24 h, the morphological changes were observed, and then cell viability was analyzed by MTT assay (b). c Chi3L1, p53, p21, BAX, cleaved caspase 3, phoshp-STAT3, STAT3, and β-actin were analyzed by Western blotting. d After the lung cancer cells were transfected with Chi3L1 siRNA for 24 h, cell lysates were immunoprecipitated with anti-control IgG or anti-p53, and then the cell lysates (bottom) and immunoprecipitants (top) were then analyzed by immunoblotting with anti-ubiquitin and anti-p53 antibodies. e The disease connections of Chi3L1 (Top) and p53 (Bottom) were analyzed by publicly available Disease-Connect from the mechanism-based disease-disease connections (http://disease-connect.org). f The signaling network among Chi3L1, p53, and related signals and was predicted using GeneMANIA (https://genemania.org/). Data shown represent mean ± SEM. *, p < 0.05 compared to con siRNA