Fig. 1

Expression of sialylated N-glycans in five bladder cancer or epithelial cell lines. N-glycans of HCV29, KK47, YTS-1, SV-HUC-1, and J82 cells were converted with acetohydrazides and released. N-glycans from KK47 cells were derivatized using [¹³C₆]-aniline, and their expression level was defined as 1 for comparative analytical purposes. N-glycans from other cells were derivatized using [¹²C₆]-aniline. The annotated N-glycans were detected in at least three biological replications. a Sialylated N-glycans in the five cell lines were annotated. b Relative expression of sialylated N-glycans was quantified and presented as mean ± SD from triplicate experiments. c Sialic acids in bladder cancer tissue (orange) and the adjacent tissue (green) form patients with bladder cancer. Lectin blot analysis was performed using biotinylated lectin SNA and MAL-II to detect sialic acids modification in clinic samples. Signals were detected using a Vectastain ABC kit (Vector Laboratories; Burlingame, CA, USA) as described in Supplementary information / Methods. β-Actin expression was used as a loading control