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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Cell death induced by the ER stressor thapsigargin involves death receptor 5, a non-autophagic function of MAP1LC3B, and distinct contributions from unfolded protein response components

Fig. 3

Tg-induced cell death requires PERK, ATF4 and CHOP. LNCaP cells were transfected for 2 d with the indicated siRNAs (siCtrl = non-targeting control siRNA), employing two different siRNA oligoes for each target (designated by − 1 and − 2). Subsequently, cells were treated with 0.02% DMSO (“DMSO”, also transfected with siCtrl) or 100 nM Tg in the absence of presence of 100 nM PERK inhibitor GSK2606414 (PERKi, also transfected with siCtrl), and the additional presence of 2.5 μg/ml propidium iodide in all conditions to stain dead cells. Cell death was monitored and quantified with the IncuCyte ZOOM as described in Materials and Methods, and displayed as relative values normalized to those obtained in the siCtrl+Tg condition after 48 h of treatment (mean value set to 1). Mean ± SEM from 4 independent experiments. Dots represent individual values, with a separate color for each experiment. ***P < 0.001, One-way ANOVA compared to the Tg + siCtrl condition

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