Fig. 2From: mTOR may interact with PARP-1 to regulate visible light-induced parthanatos in photoreceptorsPARP-1 and mTOR are activated and interact during light exposure-induced damage. a, f Cells were cultured under light/dark conditions for 72 h, followed by cell lysing and analysis with Western blot. β-ACTIN was used as an internal control. Scramble: cells were transfected with scrambled shRNA as a negative control; mTOR KD: mTOR knockdown; PARP-1 KD: PARP-1 knockdown; Lt: 1500 lx light exposure for 72 h. b–e, g–i Quantitative analysis of target protein levels related to β-ACTIN. All experiments were repeated in triplicate, and the results are shown as the means ± SEM (**: P < 0.01, ***: P < 0.001)Back to article page