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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: mTOR may interact with PARP-1 to regulate visible light-induced parthanatos in photoreceptors

Fig. 1

mTOR/PARP-1 knockdown protects photoreceptor cells against light injury. a, b 661 W cells were transfected with specific shRNAs targeting mTOR/PARP-1 genes, and cell lysates were analyzed by Western blot. β-ACTIN was used as an internal control. Scramble: cells were transfected with scrambled shRNA as a negative control; mTOR KD: cells with mTOR knockdown; PARP-1 KD: cells with PARP-1 knockdown. c, e Cells were exposed to 1500 lx light for 72 h and cell mortality was determined by PI/Hoechst staining. Scale bar = 100 μm. d, f Quantitative analysis of cell mortality. Lt: light exposure for 72 h. All experiments were repeated in triplicate, and the results are shown as the means ± SEM (n = 3, ***: P < 0.001, ns: not statistically significant)

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