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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Deletion of low-density lipoprotein-related receptor 5 inhibits liver Cancer cell proliferation via destabilizing Nucleoporin 37

Fig. 2

Knockdown of LRP5 destabilizes NUP37. a Western blot showing the expression of NUP37 following siRNA knockdown of LRP5 (three distinct siRNA targeting different segment of LRP5 ORF) or LRP6 or β-catenin for 48 h (left). si-hNUP37, positive control. n = 3. Densitometry analysis normalized with the loading control, GAPDH (right). b Real-time PCR analysis following siRNA knockdown of LRP5 (three distinct siRNA targeting different segment of LRP5 ORF), showing no significant changes in mRNA expression of NUP37 (left), si-hNUP37, positive control. n = 3. and significant down-regulation of LRP5 mRNA expression (right). n = 3. c Western blot analysis demonstrating that over-expression of LRP5 promotes stabilization of nuclear NUP37 in dose dependent manner, even at relatively low dosage. GAPDH, loading control

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Cell Communication and Signaling

ISSN: 1478-811X