Fig. 4

Uptake of EVs from T98G cells by astrocytes. EVs from T98G cells were stained green with PKH67 and placed onto astrocytes. a The astrocytes were fixed at 1 h, 12 h, and 24 h, and their cytoskeleton stained red using an Alexa Fluor 555-tagged phalloidin; their nuclei were visualized as blue with DAPI. All conditions showed uptake of EVs by astrocytes by 24 h. b A 2-D projection of a 3-D model of an astrocyte receiving EVs was obtained using z-stacks from confocal imaging. Showing the astrocyte from the side, the image demonstrates that EVs are internalized into the cells