Fig. 3

In situ detection of CD147 in glioblastoma cells. CD147 localization in glioblastoma cells was followed via immunofluorescence. a T98G cells were γ-irradiated (8 Gy) and then fixed at 15 min, 30 min, 1 h, 6 h, and 24 h after irradiation. The CD147 was stained green (Alexa Fluor 488), with the nuclei visualized as blue (DAPI). b T98G, U-87 MG, and U-118 MG cells were fixed, stained, and imaged by confocal microscopy. Antibodies were used to stain CD147 green (Alexa Fluor 488) and CD63 red (Alexa Fluor 568). The cytoskeleton was stained using phalloidin (Alexa Fluor 647), which was visualized using greyscale. The nuclei were stained blue using DAPI