Fig. 6

miR-6855 binds to the inter-AUG sequence of the PRDX5A transcript and regulates nuclear localization of PRDX5A. a Sequence alignment of miR-6855-3p in the 3′-to-5′ orientation with the PRDX5 mRNA sequence in the 5′-to-3′ orientation to predict miR-6855-3p binding site. b The RNA hybrid structure from RNAhybrid analysis between miR-6855-3p and PRDX5-interAUG (54-75 bp) is shown, with minimum free energy; mfe = − 24.1 kcal/mol. c Schematic of the binding of miR-6855-3p between the two in-frame start codons of the PRDX5 mRNA to illustrate the difference between SPRDX5 (lacks MLS) and LPRDX5 (contains MLS). d and e qPCR to determine relative RNA levels for USP20 (d) and miR-6855-3p (e) after 1 h of H₂O₂ treatment followed by 5 h of recovery time. f Luciferase reporter assay showing the effect of SFP treatment on miR-6855-3p-specific and USP20-specific promoters g Luciferase reporter assay to determine the effect of miR-6855-3p mimic on the wildtype and mutant inter-AUG sequence of the PRDX5A transcript