Fig. 2

Expression and nuclear localization of PRDX5 increases in dividing cells. a Immunoblots of BRCA2 and PRDX5 in quiescent and dividing BC cells. Lysates (30 μg of protein/lane) were resolved on a 5–12% SDS-PAGE. GAPDH served as the loading control. b Densitometry of western blots such as those in a to evaluate the relative levels of BRCA2 and PRDX5 in dividing and quiescent cells. Results are mean ± SE (n = 3). *** indicates statistical significance, p < 0.0001. c Analysis of PRDX5 in the cytosolic and nuclear fractions (CF and NF, respectively) by western blotting. CF and NF were isolated from synchronized BT549 cells at the quiescent (Q) and dividing (D) stages. GSK3β served as a normalizing control since it is equally distributed in both fractions. d Densitometry of western blots such as those in c showing relative levels of nuclear and cytosolic PRDX5 in quiescent and dividing cells compared to levels of cytosolic PRDX5 in quiescent cells. Results are mean ± SE (n = 3). The difference in PRDX5A levels is statistically significant (p < 0.001)