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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: The role of the redox/miR-6855-3p/PRDX5A axis in reversing SLUG-mediated BRCA2 silencing in breast cancer cells

Fig. 1

Human BRCA2 gene silencer is de-silenced in dividing SLUG-positive BT549 cells. a A schematic of the human BRCA2 promoter and silencer. A detailed illustration of the silencer shows the E2-box (SLUG binding site) and putative PRDX5 binding site (underlined). The A-box and B-box represent sequences required for translation initiation by RNA polIII. The numbers shown are with respect to the BRCA2-transcription start sites. b CLUSTAL-Omega-(1.2.4) sequence alignment of the human BRCA2 silencer with that of full-length Alu element. The putative E2-box, A-box, and B-box are labeled. The putative SPRDX5 binding site is highlighted. c Activity of the BRCA2 promoter in the presence of the silencer in SLUG-positive BT549 and SLUG-negative MDA-MB-468 cells. d Activity of the BRCA2 promoter in the presence of the silencer in quiescent (non-dividing) and dividing SLUG-positive MDA-MB-231 cells. Data are presented as mean ± SE (n = 6). RLU, relative light units. The difference between the luciferase activity in the quiescent and dividing cells is statistically significant (p < 0.001)

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Cell Communication and Signaling

ISSN: 1478-811X