Fig. 6From: NQO1 potentiates apoptosis evasion and upregulates XIAP via inhibiting proteasome-mediated degradation SIRT6 in hepatocellular carcinomaReintroduction of SIRT6 antagonizes NQO1 deficiency-induced apoptosis in vivo. NQO1 knock-out cells Huh-7 cells, parental cells or NQO1 knock-out cells stably overexpressing SIRT6 were injected orthotopically into 4-week-old nude mice. At 5 weeks after implantation, the animals were sacrificed, and the tumor masses were excised. NQO1 knock-out groups were injected with or without MG132 (0.05 mg/kg, intraperitoneally, daily) for 30 days starting 5 days after the injection of HCC cells. a Representative images of tumors formed (Left panel) and tumor volume in different groups of nude mice (Right panel). b Representative images of immunohistochemical staining of NQO1, SIRT6, XIAP and Cleaved PARP in tumor xenografts counterstaining with hematoxylin. Magnification, × 400Back to article page