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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: NQO1 potentiates apoptosis evasion and upregulates XIAP via inhibiting proteasome-mediated degradation SIRT6 in hepatocellular carcinoma

Fig. 5

NQO1 stabilizes SIRT6 protein via inhibiting ubiquitin-mediated proteasome degradation. a Reciprocal co-immunoprecipitation of NQO1 and SIRT6. b The co-immunoprecipitation was performed in PLC/PRF/5 and Huh-7 cells co-transfected with NQO1 and SIRT6 constructs. c Immunoblotting analysis for SIRT6 in PLC/PRF/5 and Huh-7 cells ectopically expressing NQO1 treated with NADH (100 μM) for 4 h. d-e The interaction between NQO1 and SIRT6 was examined by co-immunoprecipitation assay. NQO1 and SIRT6 constructs were co-transfected into PLC/PRF/5 cells. At 48 h after transfection, cells were treated with NADH (100 μM) (d) and dicoumarol (400 μM) for 4 h (e). f Reciprocal co-immunoprecipitation assay was performed in PLC/PRF/5 cells using anti-NQO1, anti-SIRT6 and anti-26S antibody respectively, and immunoblotted with the indicated antibody

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Cell Communication and Signaling

ISSN: 1478-811X