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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: NQO1 potentiates apoptosis evasion and upregulates XIAP via inhibiting proteasome-mediated degradation SIRT6 in hepatocellular carcinoma

Fig. 4

NQO1 knock-down/knock-out decreases the protein stability of SIRT6. a Real-time PCR for SIRT6 mRNA level in NQO1 knock-down PLC/PRF/5 and NQO1 knock-out Huh-7 cells. Data are mean ± SEM of n = 3 independent experiments. *p < 0.05. b Immunoblotting analysis for SIRT6 in NQO1 knock-down/knock-out cells. c Immunoblotting analysis for SIRT6 in PLC/PRF/5 and Huh-7 cells treated with dicoumarol (200 μM or 400 μM) for 4 h. d Immunoblotting analysis for SIRT6 in NQO1 knock-down/knock-out cells treated with 10 μg/ml cycloheximide at the indicated times. e Immunoblotting analysis for SIRT6 in NQO1 knock-down/knock-out cells treated with MG132 (10 μM) for 8 h. f Immunoblotting analysis of SIRT6 in NQO1-knock-down/knock-out cells treated with PYR-41 (50 μM) for 6 h. g SIRT6 ubiquitination was examined by immunoprecipitation assay in PLC/PRF/5 and Huh-7 cells

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Cell Communication and Signaling

ISSN: 1478-811X