Fig. 3

SIRT6 regulates phosphorylation and degradation of XIAP via deacetylation of AKT. a Immunoblotting analysis for AKT and phospho-AKT (pSer473) in SIRT6 knock-down cells. b Co-immunoprecipitation of AKT and SIRT6. Endogenous SIRT6 was immunoprecipitated using SIRT6 antibody and immunoblotted by AKT antibody (Left panel). The co-immunoprecipitation assay was also performed with the indicated antibodies in cells ectopically expressing SIRT6 and XIAP (Right panel). c The acetylation level of AKT was examined by immunoprecipitation assay with the anti-AKT antibody and probed with anti-acetylated-lysine antibody. d The interaction between AKT and XIAP was examined by the reciprocal co-immunoprecipitation in cells co-transfected with AKT and XIAP constructs. e Immunoblotting analysis for AKT, phosphor-AKT (pSer473), XIAP and phosphor-XIAP (pSer87) in SIRT6-overexpressing PLC/PRF/5 cells treated with AKT inhibitor MK2206 (10 μM) for 24 h. f Immunoblotting analysis for AKT, phosphor-AKT (pSer473), XIAP and phosphor-XIAP (pSer87) in SIRT6 knock-down cells transfected with AKT or empty vector