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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: NQO1 potentiates apoptosis evasion and upregulates XIAP via inhibiting proteasome-mediated degradation SIRT6 in hepatocellular carcinoma

Fig. 2

SIRT6 stabilizes XIAP protein from ubiquitin-mediated proteasome degradation. a Immunoblotting analysis for XIAP, phospho-XIAP (pSer87) and SIRT6 in SIRT6 knock-down cells. PLC/PRF/5 and Huh-7 cells were transduced with lentivirus expressing SIRT6-target shRNA (shSIRT6–1 and shSIRT6–2) and shCont. The expression of XIAP and phosphor-XIAP were analyzed 72 h after lentivirus transduction. b The protein levels of XIAP was examined by immunoblotting analysis. SIRT6 knock-down cells were incubated with 10 μg/mL cycloheximide and then harvested at the indicated times. c Immunoblotting analysis for XIAP in SIRT6 knock-down cells treated with proteasome inhibitor MG132 (10 μM) for 8 h. d XIAP ubiquitination were examined by immunoprecipitation assay. HA-Ub and XIAP were co-transfected into SIRT6 silencing or control cells; 72 h after transfection, cells were treated with MG132 for 8 h prior to be harvested to prevent proteasomal degradation

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