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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: NQO1 potentiates apoptosis evasion and upregulates XIAP via inhibiting proteasome-mediated degradation SIRT6 in hepatocellular carcinoma

Fig. 1

NQO1 increases XIAP phosphorylation via AKT activation. a Immunoblotting analysis for AKT and phosphor-AKT (pSer473) in NQO1 knock-down/knock-out cells or NQO1 knock-out cells transfection of vector expressing NQO1. b Immunoblotting analysis for AKT, phosphor-AKT (pSer473), XIAP, and phosphor-XIAP (pSer87) in NQO1 knock-out cells. NQO1 cells were transfected with plasmid expressing NQO1 and then treated with AKT inhibitor MK2206 (10 μM) for 24 h. c Immunoblotting analysis for AKT, phosphor-AKT (pSer473), XIAP and phosphor-XIAP (pSer87) in NQO1 knock-down/knock-out cells transfected with AKT or empty vector. d-e Trypan blue exclusion assay (d) and flow cytometry (e) were performed to analyze the NQO1-depleted cells transfected with AKT. Data are mean ± SEM of n = 3 independent experiments

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