Fig. 2

Let-7 represses PD-L1 glycosylation but enhances PD-L1 degradation. a the PD-L1 glycosylation modulation in FADU and Cal27 cells examined by addition with PNGase F and western blot analysis. b, after overexpression of let-7a and let-7b in FaDu and cal 27, the content and molecular weight of PD-L1 were detected by western blot c the degradation rates of PD-L1 was examined by western blot analysis after adding with CHX and tunicamycin, respectively. d the degradation rates of PD-L1 was examined by western blot analysis after treatment of over-expressed let-7a and let-7b, respectively. * p < 0.05 compared with the control group. The results were measurement data and were expressed as mean ± standard deviation. Data at different time points among multiple groups were analyzed by repeated measure ANOVA, with post hoc test conducted using Bonferroni. The experiment was repeated three times. PD-L1, programmed death-ligand 1; CHX, cycloheximide; IP, immune precipitation; ANOVA, analysis of variance