Fig. 4
From: Expression of E-cadherin and specific CXCR3 isoforms impact each other in prostate cancer
Overexpression of CXCR3 variants diametrically regulate E-cadherin. In (a-c), transient transfected DU-H cells with empty or CXCR3-A plasmids. a Representative immunoblot of E-cadherin, CXCR3-B and CXCR3, GAPDH as loading control. b Semi-quantification of protein expression levels by immunoblot band densitometry, student t-test, *, p < 0.05, n = 4. c Quantitative real-time PCR analysis, data shown as ratio of mRNA levels of CXCR3-A/empty plasmids transfections. One representative experiment is presented with 4 independent repeats. In (d-e), transient transfected DU-L cells with empty or CXCR3-B plasmids. d Representative immunoblot of E-cadherin, CXCR3-B and CXCR3, GAPDH as loading control. e Semi-quantification of protein expression levels by western blot bands, student t-test, *, p < 0.05, n = 4. f) Quantitative real-time PCR analysis, data shown as ratio of mRNA levels of CXCR3-B/empty plasmids transfections. One representative experiment of 4 independent repeats is presented