Fig. 3
From: Expression of E-cadherin and specific CXCR3 isoforms impact each other in prostate cancer
Reduction in E-cadherin decreased CXCR3 expression and membrane presentation. In (a-g), DU145 E-cadherin high sub-line with stable expression of control shRNA (DH-shCtrl) or E-cadherin shRNA (DH-shEcad). a Immunofluorescence staining of E-cadherin (green) and DAPI (blue). Bar = 50μm. b Immunoblot of E-cadherin expression, GAPDH as loading control. c Quantitative real-time PCR analysis of mRNA levels of CXCR3-A, CXCR3-B and E-cadherin; normalized to GAPDH. In (d-g), flow cytometry assays of whole cell level of CXCR3-B (d), externally-accessible CXCR3-B (e), whole cell level of total-CXCR3 (f), externally-accessible total-CXCR3 (g). The Geometric Mean Fluorescence Intensity (MFI) or percentage of M1 among the gated cells is on the right. Student t-test, *, p < 0.05; ****, p < 0.0001; N.S, non-specific. One representative experiment of at least 3 independent repeats is presented in all panels